UV spectrophotometer working principle and use

The basic working principle of UV spectrophotometer:

The basic working principle of an ultraviolet spectrophotometer is similar to that of an infrared spectrometer. A certain frequency of ultraviolet-visible light is used to irradiate the analyzed organic material, causing the valence electron transition in the molecule, which will be selectively absorbed. A group absorbs the spectrum as a function of wavelength and reflects the characteristics of the sample. In the UV-Vis range, for a specific wavelength, the degree of absorption is proportional to the concentration of the component in the sample, so the measurement spectrum can be qualitatively analyzed, and can also be performed according to the comparison of the absorption with a known concentration of the standard. Quantitative analysis.

UV spectrophotometer main use:

The UV-visible spectrometer involves a wavelength range of 0.2--0.8 microns (corresponding to wavenumbers of 50,000 to 12,500 cm-1), which has been widely used in organic chemistry research. It is commonly used for substance identification, purity check, and organic molecular structure research. In the quantitative aspect, the content of each component in the more complex structures and mixtures can be determined. It can also determine the dissociation constant of the substance, the stability constant of the complex, the identification of the molecular weight of the substance and the indicated end points in the microtitration, and the high performance liquid chromatography. As a detector and so on.